5-Amino-1MQ

Buy 5-Amino-1MQ (50mg vials) from Peptides Partners

Laboratories and academic institutions seeking high-grade biochemical tools for metabolic investigations can buy 5-Amino-1MQ in robust 50mg vial formats directly through Peptides Partners. Formulated specifically to meet the rigorous demands of preclinical in vitro and in vivo models, our 5-Amino-1MQ supplies provide qualified investigators with a dependable compound for examining energy expenditure pathways, enzyme dynamics, and cellular differentiation markers. As part of our comprehensive portfolio of research peptides and small-molecule modulators, each vial undergoes strict quality control standards to ensure chemical identity and consistency across all experimental phases.

5-Amino-1MQ (5-amino-1-methylquinolinium) has emerged as an indispensable reagent within the metabolic research domain due to its distinct pharmacological profile as a selective, membrane-permeable enzyme inhibitor. When you buy 5-Amino-1MQ from Peptides Partners, your laboratory receives lyophilized material packaged in specialized borosilicate glass vials designed to minimize environmental contamination, protect against moisture degradation, and facilitate standardized reconstitution protocols. Whether your research group is running high-throughput cellular screening or long-term comparative metabolic trials, Peptides Partners delivers premium research peptides and related biochemicals configured for reproducible empirical outcomes.

What is 5-Amino-1MQ?

5-Amino-1MQ is a small-molecule quinolinium derivative structurally identified as 5-amino-1-methylquinolin-1-ium. Developed through medicinal chemistry research focused on small-molecule regulators of cellular metabolism, it functions primarily as a selective and potent inhibitor of nicotinamide N-methyltransferase (NNMT). In standard biochemical classification, while often grouped alongside metabolic research peptides and synthetic cellular regulators, 5-Amino-1MQ possesses a distinct synthetic small-molecule scaffold optimized for passive membrane permeability and high specificity toward the NNMT catalytic domain.

The target enzyme, NNMT, is a key cytosolic enzyme that methylates nicotinamide (vitamin B3) using S-adenosylmethionine (SAM) as the universal methyl donor. This biochemical reaction yields 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH). Because NNMT operates at the intersection of cellular methylation status and the salvage pathway for nicotinamide adenine dinucleotide (NAD+), its inhibition by 5-Amino-1MQ creates a profound shift in intracellular resource allocation. By blocking the consumption of nicotinamide by NNMT, 5-Amino-1MQ diverts nicotinamide toward the NAD+ salvage pathway, facilitating the biosynthesis of intracellular NAD+ while simultaneously preserving the cellular pool of SAM for vital histone and DNA methyltransferase reactions.

Historically identified as an overexpressed target in various metabolic and physiological alterations, NNMT has become a focal point for understanding cellular energetics. 5-Amino-1MQ was engineered specifically to address the limitations of prior, non-selective NNMT inhibitors that suffered from poor cell permeability or broad off-target activity against other methyltransferases. With a low molecular weight and optimized charge distribution, 5-Amino-1MQ rapidly penetrates plasma membranes without requiring specialized delivery vectors, making it an extraordinarily efficient tool for bench research targeting metabolic biochemistry, epigenetic regulation, and cellular aging models.

Research effects studied

Over the past several years, scientific publications have investigated 5-Amino-1MQ across an array of preclinical models, examining its mechanistic impacts on metabolic rate, adiposity, muscle biology, and cellular signaling networks. Because NNMT is predominantly expressed in adipose tissue, liver tissue, and skeletal muscle stem cells, these target areas represent the majority of published investigations.

• Cellular NAD+ Kinetics and Mitochondrial Metabolism: Studies in cultured adipocytes and primary cell models have demonstrated that the introduction of 5-Amino-1MQ leads to an elevation of intracellular NAD+ levels. By preventing the terminal methylation of nicotinamide into 1-MNA, cells preserve the substrate required for nicotinamide phosphoribosyltransferase (NAMPT) to produce NAD+. Elevated NAD+ subsequently activates sirtuin pathways, specifically SIRT1, a crucial NAD+-dependent deacetylase that stimulates mitochondrial biogenesis, enhances cellular oxygen consumption rates (OCR), and promotes mitochondrial electron transport chain efficiency.
• Adipogenesis and Energy Homeostasis: Preclinical research in rodent models subjected to diet-induced obesity has shown that administration of NNMT inhibitors such as 5-Amino-1MQ correlates with reductions in body adiposity without a decrease in food intake. In vitro analyses suggest that 5-Amino-1MQ directly downregulates lipogenic gene expression, alters lipid droplet accumulation in mature adipocytes, and elevates resting energy expenditure via uncoupling mechanisms and substrate cycling.
• Skeletal Muscle Function and Satellite Cell Activation: Research conducted on aging and injured skeletal muscle models has revealed compelling interactions between NNMT inhibition and muscle regeneration. In aged murine systems, NNMT expression is significantly elevated in muscle satellite cells (stem cells), which limits their capacity for self-renewal and proliferation. Preclinical investigations have observed that treatment with 5-Amino-1MQ restores the proliferative and regenerative potential of these aged satellite cells, leading to augmented muscle fiber cross-sectional area, improved contractile force generation, and accelerated structural repair following induced myofiber damage.
• Epigenetic Remodeling via Methyl Donor Conservation: Beyond energy pathways, the role of NNMT as a consumer of SAM implies that its activity limits methyl group availability for chromatin modifications. Preclinical investigations have analyzed how 5-Amino-1MQ modulates the SAM/SAH ratio in target cells, noting significant changes in histone methylation marks (such as H3K4me3 and H3K27me3). Researchers frequently utilize 5-Amino-1MQ to observe how these epigenetic shifts influence transcriptional reprogramming across metabolic and structural gene clusters.
• Glucose Uptake and Insulin Signaling Pathways: In various metabolic cell culture assays, 5-Amino-1MQ has been studied for its capacity to alter insulin-stimulated glucose uptake. Preclinical data highlight enhanced phosphorylation of key signaling intermediates, including Akt, and improved translocation of glucose transporter type 4 (GLUT4) to the plasma membrane under metabolic stress conditions, providing a foundation for ongoing research into cellular insulin sensitivity.

How to use in the laboratory

To ensure consistent and reproducible experimental results, laboratory personnel must follow standard operating procedures when preparing and handling 5-Amino-1MQ. The compound is supplied as a sterile, lyophilized solid in 50mg single-use or multi-aliquot glass vials, requiring precise reconstitution and handling protocols.

• Reconstitution Methodology: 5-Amino-1MQ exhibits solubility in standard aqueous laboratory solutions as well as specialized organic solvents. Depending on downstream experimental design:
  • For aqueous applications, reconstitution can be conducted using sterile, laboratory-grade water or buffered saline (such as sterile phosphate-buffered saline, PBS, pH 7.4). Slowly introduce the solvent using an automated pipette directed along the interior glass wall of the vial to prevent aerosolization.
  • For high-concentration master stock solutions, anhydrous dimethyl sulfoxide (DMSO) may be employed. Ensure the final concentration of DMSO in subsequent cell culture media or assay systems remains well below established cellular cytotoxicity thresholds (typically <0.1% v/v).
  • Gently swirl or rock the vial until the solid is completely dissolved. Avoid excessive vigorous vortexing, which can generate bubbles or promote shear stress on molecular complexes.
• Aseptic Laboratory Handling: Reconstitution should invariably occur inside a certified Class II Biosafety Cabinet (laminar flow hood) to preserve solution sterility. Technicians must use sterile barrier pipette tips, appropriate PPE (nitrile gloves, lab coat, safety spectacles), and ensure all contact surfaces are decontaminated with 70% isopropyl alcohol prior to work.
• Aliquoting Protocols: Following dissolution, stock solutions should be divided immediately into sterile, low-binding microcentrifuge tubes to create single-use aliquots. This practice eliminates repetitive freeze-thaw cycles, which can cause gradual chemical degradation and loss of functional potency.
• Laboratory Record-Keeping: Precise documentation is critical for verifiable empirical research. Technicians should systematically record the date of reconstitution, solvent type, batch or lot number, calculated stock concentration (e.g., mM or mg/mL), storage temperature, and the operator’s initials. All containers must be marked with hazardous chemical identification tags in accordance with institutional laboratory safety guidelines.

Pack sizes and price per mg

Peptides Partners supplies 5-Amino-1MQ in standardized 50mg vials across three distinct pack sizes. Tailored to accommodate varying project budgets and research scales, these options allow laboratories to optimize costs based on experimental volume.

By offering tiered pricing that decreases to approximately $0.52 per milligram on the 10-vial option, Peptides Partners enables academic laboratories and commercial entities to plan long-term, high-replicate screening campaigns with economic predictability and uncompromised product standardisation.

Storage and handling

Preserving the integrity of 5-Amino-1MQ requires careful adherence to chemical storage conditions throughout the lifecycle of the product. The stability of the solid and liquid forms depends heavily on temperature, atmospheric moisture, and ambient light exposure.

Lyophilized Powder: In its unopened, lyophilized state, 5-Amino-1MQ should be stored at -20°C for routine medium-term laboratory use. For long-term archiving extending past 12 months, storage at -80°C in an ultra-low temperature freezer is recommended. The vials contain an inert internal atmosphere; therefore, keep the rubber stopper and crimp seal fully intact until immediate use. Prior to reconstituting, allow the frozen vial to equilibrate to room temperature inside a desiccator or sealed container for 20 to 30 minutes. Opening cold vials directly in humid ambient air will cause rapid moisture condensation inside the container, which can accelerate hydrolytic degradation.

Reconstituted Solutions: Aqueous solutions of 5-Amino-1MQ are generally stable at 4°C for short experimental windows (typically up to 7 days). For storage extending beyond one week, stock solutions should be divided into single-use aliquots and frozen at -20°C or -80°C. Protect reconstituted material from direct light sources by utilizing amber vials or wrapping standard microcentrifuge tubes in aluminum foil. Avoid repeated freeze-thaw cycles, as molecular stress can alter solubility profiles and compound efficacy. Discard any solution showing precipitation, cloudiness, or discoloration.

Quality and documentation

At Peptides Partners, product consistency and chemical precision form the foundation of our catalog. We recognize that rigorous scientific discovery demands analytical transparency, and every batch of our research peptides and metabolic agents undergoes comprehensive physical and chemical verification.

Our quality verification processes employ High-Performance Liquid Chromatography (HPLC) to verify chromatographic purity, ensuring minimal synthesis-related impurities or residual organic intermediates. Mass Spectrometry (MS) is simultaneously utilized to confirm exact molecular identity and molecular weight parameters against theoretical standards. Every batch manufactured is traceable through dedicated lot numbering systems, ensuring that laboratories receive compounds that align with strict analytical criteria.

Qualified investigators can access lot-specific Certificates of Analysis (CoA) detailing empirical analytical results. This level of quality verification guarantees that research conducted with materials from Peptides Partners can be documented with full regulatory and institutional compliance, facilitating verifiable, peer-reviewed scientific contributions.

Frequently asked questions

What is the primary biochemical target of 5-Amino-1MQ in research?

5-Amino-1MQ is a selective, cell-permeable small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). NNMT is an intracellular enzyme responsible for catalyzing the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH). By inhibiting NNMT, 5-Amino-1MQ allows researchers to interrogate the downstream metabolic, energetic, and epigenetic pathways regulated by this key enzymatic junction.

How does 5-Amino-1MQ influence cellular NAD+ levels?

Under normal conditions with active NNMT, intracellular nicotinamide is continuously methylated and cleared via 1-MNA formation. When 5-Amino-1MQ inhibits NNMT, nicotinamide is preserved and channeled into the NAD+ salvage pathway via the enzyme NAMPT. This enzymatic redirection increases intracellular concentrations of NAD+, subsequently stimulating NAD+-dependent signaling pathways, including sirtuins (SIRT1) and mitochondrial oxidative phosphorylation.

Can 5-Amino-1MQ be dissolved in aqueous buffers, or is DMSO required?

5-Amino-1MQ exhibits good solubility in aqueous buffers, including sterile bacteriostatic water, sterile deionized water, and phosphate-buffered saline (PBS). However, for high-concentration stock preparations or specific high-throughput screening applications, researchers may opt to dissolve the compound in sterile DMSO. When using DMSO, care must be taken to ensure the final solvent concentration in experimental assay environments remains low enough to avoid solvent-induced cellular toxicity.

What standard laboratory safety precautions apply when handling 5-Amino-1MQ?

5-Amino-1MQ is strictly designated for laboratory research use and must not be administered to humans or animals outside of controlled experimental frameworks. When handling the solid or reconstituted solution, technicians must wear personal protective equipment, including a laboratory coat, chemical-resistant nitrile gloves, and safety glasses. All open handling should occur inside a certified chemical fume hood or biosafety cabinet to prevent accidental inhalation, ocular exposure, or dermal contact. Dispose of all waste materials in accordance with local and institutional biohazard and chemical disposal guidelines.

Related Peptides Partners research peptides

AOD-9604 (10mg vials); Combo Vial: CJC-1295 no DAC/Ipamorelin (20mg vials); Kisspeptin (10mg vials).

5-Amino-1MQ lot documentation

Certificates of analysis are matched to the actual lot supplied with your order. Email sales@peptidespartners.co with the product name and pack size to request the current lot's purity, identity and screening documents before purchase.

Storage

Research peptides are typically supplied lyophilized (freeze-dried), which keeps them stable for shipping and storage. Store unopened vials cool, dry and away from light; for long-term storage, a freezer is generally recommended.

After reconstitution, keep vials refrigerated and use sterile laboratory technique. Follow the handling guidance supplied with your lot documentation.

Short name

5-Amino-1MQ (50mg vials)

Vial strength

50mg vials

Pack sizes

100mg pack, 250mg pack, 500mg pack

Pack count options

2, 5, 10 vial packs

Category

Metabolics

SKU

PP-61420

Compliance

Research Use Only. Not for use in diagnostic tests.